Journal: Journal of the American Chemical Society

Article Title: Light-Driven Intraoctahedral Halide Isomerization in Two-Dimensional Mixed Halide Perovskites

doi: 10.1021/jacs.5c15542

Figure Lengend Snippet: Microscopic structure–property correlation of of layered BA 2 PbBr 2 I 2 thin crystals. (a) Crystal structure of 2D BA 2 PbBr 2 I 2 from SCXRD data measured at 100 K and corresponding schematics showing 68% Br at B-site and 68% I at T-site. (b) Single PL spectra for BA 2 PbBr 2 I 2 without (gray curve) and with photoexcitation (blue curve). (c) Hyperspectral PL maps of a thin BA 2 PbBr 2 I 2 single crystal. The top and bottom images represent before and after photoexcitation by a 405 nm CW laser for 5 min. The central wavelength of the PL maps is 500 nm, which is the iodide-rich phase wavelength window. (d) Schematic illustration of photoisomerization for 2D BA 2 PbBr 2 I 2 . Note: in this diagram, all I atoms are positioned at T-site for demonstrating the halide switching purpose. (e) Schematic illustration of the random halide distribution in a 3D MAPbBr x I 3– x mixed halide perovskite network. (f) Single PL spectra for MAPbBr x I 3– x without (gray curve) and with photoexcitation (blue curve). (g) Hyperspectral PL maps of a thin MAPbBr x I 3– x single crystal. The top and bottom images represent before and after photoexcitation by a 405 nm CW laser with an excitation intensity of 80 mW/cm 2 for 5 min. The central wavelength of the PL maps is 750 nm, which is the iodide-rich phase wavelength window. (h) Schematic illustration of light-induced halide segregation for 3D MAPbBr x I 3– x . (i, k) Absorptance maps measured on a thin BA 2 PbBr 2 I 2 crystal before (i) and after excitation (k). The maps in (i) and (k) are taken at the central wavelength of 424 nm. The photoexcitation region is shown in the dashed area. (j) Optical image presenting a thin BA 2 PbBr 2 I 2 crystal where the central squared region is the photoexcited region. (l) Absorptance spectra before and after photoexcitation at three isolated points labeled in (i) and (k). The scale bars in all microscopic images are 20 μm.

Article Snippet: Spectrally resolved maps were acquired using an IMA hyperspectral microscope (Photon Etc.).

Techniques: Isolation, Labeling

Journal: Biomolecules

Article Title: Targeting Integrin α2 to Overcome Imatinib Resistance in Chronic Myeloid Leukemia Cells

doi: 10.3390/biom15091245

Figure Lengend Snippet: Cells were treated with increasing concentrations of E7820 (0.06, 0.125, 0.25, 0.5, 1, 2.5, 5, 7.5, 10, 15, 20, and 23 μM), and cell viability was assessed. ( A ) K562S, ( B ) K562R-ima, and ( C ) K562R+ima cells were treated with E7820 and compared to untreated controls. ( n = 4) (* p < 0.05, *** p < 0.001, **** p < 0.0001).

Article Snippet: Later, the 5 μM resistant K562R cell line was produced by gradually increasing the IMA (Santa Cruz, Dallas, TX, USA) concentration over a period of 18 months using the K562R 0.6 cells as the starting population [ ].

Techniques:

Journal: Biomolecules

Article Title: Targeting Integrin α2 to Overcome Imatinib Resistance in Chronic Myeloid Leukemia Cells

doi: 10.3390/biom15091245

Figure Lengend Snippet: Protein levels of ITGA2 were analyzed by flow cytometry. Columns indicate mean fluorescence intensity in ( A ) K562S; ( B ) K562R-ima; ( C ) K562R+ima. ( n = 4) (*** p < 0.001 and **** p < 0.0001).

Article Snippet: Later, the 5 μM resistant K562R cell line was produced by gradually increasing the IMA (Santa Cruz, Dallas, TX, USA) concentration over a period of 18 months using the K562R 0.6 cells as the starting population [ ].

Techniques: Flow Cytometry, Fluorescence

Journal: Biomolecules

Article Title: Targeting Integrin α2 to Overcome Imatinib Resistance in Chronic Myeloid Leukemia Cells

doi: 10.3390/biom15091245

Figure Lengend Snippet: Flow cytometric analysis using Annexin V-PE/7-AAD-stained K562S and K562R cells. Quantification of apoptotic populations (bar graph) in ( A ) K562S; ( B ) K562R-ima; ( C ) K562R+ima cells; ( D ) Dot plot representation of flow cytometry data. ( n = 4) (** p < 0.01, and **** p < 0.0001).

Article Snippet: Later, the 5 μM resistant K562R cell line was produced by gradually increasing the IMA (Santa Cruz, Dallas, TX, USA) concentration over a period of 18 months using the K562R 0.6 cells as the starting population [ ].

Techniques: Staining, Flow Cytometry

Journal: Biomolecules

Article Title: Targeting Integrin α2 to Overcome Imatinib Resistance in Chronic Myeloid Leukemia Cells

doi: 10.3390/biom15091245

Figure Lengend Snippet: Effects of E7820 (2.5 μM) on caspase3/7 activity in treated K562S and K562R cells compared to their untreated counterparts. ( A ) K562S; ( B ) K562R-ima; ( C ) K562R+ima cells ( n = 4) (** p < 0.01 and **** p < 0.0001).

Article Snippet: Later, the 5 μM resistant K562R cell line was produced by gradually increasing the IMA (Santa Cruz, Dallas, TX, USA) concentration over a period of 18 months using the K562R 0.6 cells as the starting population [ ].

Techniques: Activity Assay

Journal: Biomolecules

Article Title: Targeting Integrin α2 to Overcome Imatinib Resistance in Chronic Myeloid Leukemia Cells

doi: 10.3390/biom15091245

Figure Lengend Snippet: Gene expression analysis of K562S and K562R cells upon incubation with E7820 for 72 h by real-time PCR assay. Effects of E7820 treatment BAX , BAD , BIM , and BCL-2 gene expressions; ( A ) K562S; ( B ) K562R-ima; ( C ) K562R+ima cell; ( D ) Bar graph presentation of BAX/BCL-2 mRNA ratio. ( n = 4) (* p < 0.05, *** p < 0.001, **** p < 0.0001).

Article Snippet: Later, the 5 μM resistant K562R cell line was produced by gradually increasing the IMA (Santa Cruz, Dallas, TX, USA) concentration over a period of 18 months using the K562R 0.6 cells as the starting population [ ].

Techniques: Gene Expression, Incubation, Real-time Polymerase Chain Reaction

Journal: Biomolecules

Article Title: Targeting Integrin α2 to Overcome Imatinib Resistance in Chronic Myeloid Leukemia Cells

doi: 10.3390/biom15091245

Figure Lengend Snippet: Intracellular accumulation of Rho-123 was assessed by flow cytometry in K562S, K562R-ima, and K562R+ima cells. ( A ) Flow cytometry results are represented as dot; ( B ) Bar graph representation of flow cytometry data. ( n = 4) (**** p < 0.0001).

Article Snippet: Later, the 5 μM resistant K562R cell line was produced by gradually increasing the IMA (Santa Cruz, Dallas, TX, USA) concentration over a period of 18 months using the K562R 0.6 cells as the starting population [ ].

Techniques: Flow Cytometry